刘允军
职  称
  • 副研究员
电  话
  • 13693367706
电子邮件
  • liuyunjun@caas.cn
创新团队
  • 作物基因组选择育种
研 究 组
  • 植物转基因技术研究中心
研究中心
  • 作物基因与分子设计中心
教育经历
  • 2001.9-2004.7 中国农业大学 遗传学 博士 1998.9-2001.7 中国农业大学 作物遗传育种 硕士1994.9-1998.7 吉林农业大学 野生植物资源开发与利用 学士
工作经历
  • 2004 年8月至2005年7月 北京健平九星生物科技有限公司 2005年8月至2007年7月 瑞典Lund大学 博士后 2007 年12月至2008年12月 中国农业科学院作物科学研究所 助理研究员 2009 年1月至今 中国农业科学院作物科学研究所 副研究员
研究方向
  • 1)玉米遗传转化机理解析 2)玉米基因编辑
承担项目
  • 1. 国家自然基金“SMK11表观遗传调控玉米籽粒发育的分子机制”,起止时间2019.1-2022.12,经费60万元,主持人。 2. 转基因重大专项任务“玉米规模化转基因技术体系构建”,起止时间2011.1-2020.12,经费615万元,主持人。 3. 中国农科院所级统筹科研业务费“基于新型不育系的玉米杂交育种技术的建立”(S2018QY07),起止时间2018.1-2020.12,经费90万元,主持人。 4. 科技部973任务“玉米品质性状的全基因组遗传解析”,起止时间2014.1-2018.12,经费67万元,主持人。 5. 教育部留学人员基金“玉米锌指蛋白基因ZmSAP13的功能及作用机制研究”,起止时间2010.1-2010.10, 经费4万元,主持人。 6. 转基因重大专项子课题“抗病虫、抗除草剂转基因玉米新品种培育”,起止时间2011.1-2020.12,经费3935.86万元,参加,主要执行人。
主要论文
  • Ren, Z., Fan, K., Fang, T., Zhang, J., Yang, L., Wang, J., Wang, G. and Liu, Y. (2019) Maize Empty Pericarp602 Encodes a P-Type PPR Protein That Is Essential for Seed Development. Plant & cell physiology 60, 1734-1746.下载
  • Du X, Fang T, Liu Y, Huang LY, Wang XL, Zhang J, Cui YB, Zang MS, Wang GY, Fu JJ, Liu YJ. Global Profiling of Alternative Splicing in Callus Induction of Immature Maize Embryo. In Vitro Cellular & Developmental Biology - Plant, 2019 https://doi.org/10.1007/s11627-019-10024-4下载
  • Du, X., Fang, T., Liu, Y., Huang, L., Zang, M., Wang, G., Liu, Y. and Fu, J. (2019) Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation. Frontiers in plant science 10, 1633.下载
  • Chen, R., Xu, Q., Liu, Y., Zhang, J., Ren, D., Wang, G. and Liu, Y. (2018) Generation of Transgene-Free Maize Male Sterile Lines Using the CRISPR/Cas9 System. Frontiers in plant science 9, 1180.下载
  • Liu, Y., Zhang, Z., Fu, J., Wang, G., Wang, J. and Liu, Y. (2017) Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency. Frontiers in plant science 8, 1778.下载
  • Kang D, Wang CY, Xu QL, Wang GY, Liu YJ. Characterization of maize male sterile 2 mutant by phenotypic and RNA sequencing analyses.Plant Breeding, (2017) 136, 319–330下载
  • Cao, Z., Zhang, W., Ning, X., Wang, B., Liu, Y. and Li, Q.X. (2017) Development of Monoclonal Antibodies Recognizing Linear Epitope: Illustration by Three Bacillus thuringiensis Crystal Proteins of Genetically Modified Cotton, Maize, and Tobacco. Journal of Agricultural and Food Chemistry 65, 10115-10122.下载
  • Zhang, Y., Zhang, W., Liu, Y., Wang, J., Wang, G. and Liu, Y. (2016) Development of monoclonal antibody-based sensitive ELISA for the determination of Cry1Ie protein in transgenic plant. Analytical and Bioanalytical Chemistry 408, 8231-8239.下载
  • Zhang YW, Liu Y, Zhang J, Wang CY, Wang JH, Liu YJ. Assessment of transgene copy number and zygosity of transgenic maize overexpressing Cry1Ie gene with SYBR® Green qRT-PCR.In Vitro Cell.Dev.Biol.—Plant (2015) 51:125–134下载
  • Yuan Y, Qi LJ, Yang J, Wu C, Liu YJ, Huang LQ. A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco.Plant Cell Tiss Organ Cult (2015) 120:961–972下载
  • Ren ZJ, Liu Y, Kang D, Fan KJ, Wang CY, Wang GY, Liu YJ. Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants. Plant Cell Tiss Organ Cult (2015) 123:569–578下载
  • Ren ZJ, Cao GY, Zhang YW, Liu Y, Liu YJ(2015) Overexpression of a modified AM79 aroA gene in transgenic maize confers high tolerance to glyphosate. Journal of Integrative Agriculture, 414-422.下载
  • Liu, Y., Cao, G., Chen, R., Zhang, S., Ren, Y., Lu, W., Wang, J. and Wang, G. (2015) Transgenic tobacco simultaneously overexpressing glyphosate N-acetyltransferase and 5-enolpyruvylshikimate-3-phosphate synthase are more resistant to glyphosate than those containing one gene. Transgenic Research 24, 753-763.下载
  • Liu YJ, Zhang YW, Liu Y, Lu W, Wang GY. Metabolic effects of glyphosate on transgenic maize expressing a G2-EPSPS gene from Pseudomonas fluorescens.J. Plant Biochem. Biotechnol. (2015) 24(2):233–241下载
  • Zhang YW, Liu YJ, Ren Y, Liu Y, Liang GM, Song FP, Bai SX, Wang JH, Wang GY. Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize. Plant Cell Tiss Organ Cult (2013) 115:151–158下载
  • Zhang SX, Lian Y, Liu Y, Wang XQ, Liu YJ, Wang GY. Characterization of a Maize Wip1 Promoter in Transgenic Plants. Int. J. Mol. Sci. 2013, 14, 23872-23892下载
  • Yuan Y, Wu C, Liu Y, Yang J, Huang L (2013) The Scutellaria baicalensis R2R3-MYB Transcription Factors Modulates Flavonoid Biosynthesis by Regulating GA Metabolism in Transgenic Tobacco Plants. PLoS ONE 8(10): e77275.下载
  • Yuan Y, Liu Y, Wu C, Chen S, Wang Z, Yang Z, Qin SS, Huang LQ (2012) Water Deficit Affected Flavonoid Accumulation by Regulating Hormone Metabolism in Scutellaria baicalensis Georgi Roots. PLoS ONE 7(10): e42946.下载
  • Liu YJ, Yuan Y, Liu YY, Liu Y, Fu JJ, Zheng J, Wang GY. Gene families of maize glutathione–ascorbate redox cycle respond differently to abiotic stresses. Journal of Plant Physiology 169 (2012) 183– 192下载
  • Cao G, Liu Y, Zhang S, Yang X, Chen R, et al. (2012) A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants. PLoS ONE 7(6): e38718.下载
  • Liu, Y.J., Nunes-Nesi, A., Wallstrom, S.V., Lager, I., Michalecka, A.M., Norberg, F.E., Widell, S., Fredlund, K.M., Fernie, A.R. and Rasmusson, A.G. (2009) A redox-mediated modulation of stem bolting in transgenic Nicotiana sylvestris differentially expressing the external mitochondrial NADPH dehydrogenase. Plant Physiology 150, 1248-1259.下载
  • Liu, Y.J., Norberg, F.E., Szilagyi, A., De Paepe, R., Akerlund, H.E. and Rasmusson, A.G. (2008) The mitochondrial external NADPH dehydrogenase modulates the leaf NADPH/NADP+ ratio in transgenic Nicotiana sylvestris. Plant & Cell Physiology 49, 251-263.下载
授权专利
  • 1.刘允军,王国英,刘艳. 一种通过弱光培养体系快速获得转基因植物的方法 (专利号201710090814.2) 2.刘允军,任珍静,王国英. 用于提高植物耐盐性的编码玉米HKT转运蛋白的基因ZmHKT1;1a及其应用(专利号201410837383.8) 3.刘允军,任珍静,王国英. 一种玉米耐盐基因及其应用(专利号201410838479.6) 4.刘允军,张煜文, 王国英,何康来. 一种杀虫蛋白及其编码基因与应用(专利号201410591794.3) 5.刘允军,王国英,张煜文,刘艳. 融合杀虫蛋白Cry1Am、其编码基因及应用(专利号201510175515.X) 6.刘允军,王国英,刘艳. 一种农杆菌侵染玉米幼胚的方法 (专利号201310022133.4)